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產(chǎn)品名稱(chēng):MassChrom® Vitamins B1 and B6 in Whole Blood, with 96 Deep Well Plate

產(chǎn)品型號(hào):87000/DWP

產(chǎn)品報(bào)價(jià):

產(chǎn)品特點(diǎn):

供應(yīng)商:祥生興業(yè)規(guī)格:480TestsMassChrom®VitaminsB1andB6inWholeBlood,with96DeepWellPlateOrderno

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87000/DWPMassChrom® Vitamins B1 and B6 in Whole Blood, with 96 Deep Well Plate的詳細(xì)資料:

溫馨提示:僅用于科研,不可用于臨床治療

  • 供應(yīng)商:

    祥生興業(yè)

  • 規(guī)格:

    480 Tests

    MassChrom® Vitamins B1 and B6 in Whole Blood, with 96 Deep Well Plate


    Order no.: 87000/DWP
    480 Tests
    • Vitamin B1 (TPP, thiamine pyrophosphate)
    • Vitamin B6 (PLP, pyridoxal 5’-phosphate)

    Fast and easy sample preparation
    Robust method with high selectivity
    Short run time of 2.5 minutes
    Isotopically-labelled internal standard for each analyte
    With 96 deep well plates

     

    DESCRIPTION

    Clinical relevance

    Vitamin B1 (thiamine) plays an important role in deriving energy from carbohydrates and fats. The active form is thiamine pyrophosphate (TPP), a coenzyme for some enzymatic reactions in carbohydrate metabolism and for pyruvate decarboxylase. TPP is also significantly involved in the oxidative degradation of glucose. The determination of the TPP concentration is more significant than measurements of total thiamine, as by this method only the physiologically active form is taken into account.

    Vitamin B6 comprises pyridoxine, pyridoxamine and pyridoxal, as well as their phosphate derivatives. It is ingested with food and transferred via several enzymatic conversions into its active form, pyridoxal-5’-phosphate (PLP). PLP is involved in nearly one hundred different enzymatic reactions, among other things, as co-factor in amino acid metabolism, and in the formation of haemoglobin or neurotransmitters in the brain.

     

    Product advantages

    • Fast and easy sample preparation
    • Robust method with high selectivity
    • Short run time of 2.5 minutes
    • Isotopically-labelled internal standard for each analyte

     

    This Chromsystems assay enables combined determination of the active forms of vitamin B1 (thiamine pyrophosphate, TPP) and vitamin B6 (pyridoxal 5‘-phosphate, PLP) in whole blood by LC-MS/MS. Sample preparation consists of a simple protein precipitation step, with no need for derivatisation. This makes for a much faster sample preparation and high sample throughput. Analytes and potential interferences are effectively and swiftly separated within 2.5 minutes, indicating the method's high selectivity and speed. The chromatographic separation of the MassChrom® assay is fast enough to ensure a high throughput in the laboratory, but sufficiently resolving for a clear separation of the two analytes with minimal potential matrix effects. Isotope-labelled internal standards for both parameters as well as 6PLUS1® calibrators and MassCheck® controls ensure accurate and precise results. The assay is available in 2 configurations either with reaction vials or with 96 deep well plates.

    CHROMATOGRAM

    Chromatogram 87000 Vitamins B1 and B6

    TECHNICAL DATA

    Method of Analysis

    LC-MS/MS

    Number of Tests

    480

    Parameter

    Vitamin B1 (TPP, thiamine pyrophosphate), Vitamin B6 (PLP, pyridoxal 5’-phosphate)

    Analysis Time

    2.5 min

    Limit of quantification

    3.4 μg/l TPP
    2.8 μg/l PLP

    Linearity

    850 μg/l TPP
    1000 μg/l PLP

    Intraassay

    CV = 1.9–5.2 %

    Interassay

    CV = 2.5–7.1 %

    Specimen

    Whole Blood

    Sample Preparation

    • Pipette 40 μl sample/calibrator/MassCheck® control into 96 deep well plate.
    • Add 80 μl Internal Standard Mix, shake briefly.
    • Add 350 μl Precipitation Reagent, shake for 1 min and centrifuge for 5 min.
    • Transfer supernatant into collection plate.
    • Inject 20 μl into the LC-MS/MS system.

    Injection Volume

    20 μl

    Flow rate

    0.5 ml/min

    Column temperature

    30 °C

    Ionisation

    ESI positive

    MS/MS-Mode

    MRM

    Please note

    The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

    KIT COMPONENTS

    Components available separately

    Mobile Phase A


    Order no.: 87001

    DETAILS

    Mobile Phase B


    Order no.: 87002

    DETAILS

    Internal Standard Set Vitamins B1 and B6


    Order no.: 87046

    DETAILS

    Precipitation Reagent


    Order no.: 87005

    DETAILS

    Rinsing Solution


    Order no.: 87009

    DETAILS

    96 Deep Well Plates


    Order no.: 87057

    DETAILS

    Collection Plates


    Order no.: 87058

    DETAILS

    Pierceable adhesive seals for 96 Well Plates


    Order no.: 87059

    DETAILS

    ACCESSORIES

    Analytical column


    Order no.: 87100

    DETAILS

    Dilution Buffer


    Order no.: 87018

    DETAILS

    Tuning Mix Vitamins B1 and B6


    Order no.: 87015

    DETAILS

    Adjustment Reagent


    Order no.: 87019

    DETAILS

    CALIBRATORS

    Calibrators available separately

    6PLUS1® Multilevel Whole Blood Calibrator Set Vitamins B1 and B6


    Order no.: 87039

    DETAILS

    CONTROLS

    Controls available separately

    MassCheck® Vitamins B1 and B6 Whole Blood Controls


     

    DETAILS

溫馨提示:不可用于臨床治療。
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