MassChrom® Vitamins B1 and B6 in Whole Blood, with Reaction Vials |
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供應(yīng)數(shù)量： |
87 |
發(fā)布日期： |
2024/12/25 |
有效日期： |
2025/6/25 |
原 產(chǎn) 地： |
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已獲點擊： |
87 |
產(chǎn)品報價： |
 |
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溫馨提示：僅用于科研，不可用于臨床治療 - 供應(yīng)商：
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480 Tests  MassChrom® Vitamins B1 and B6 in Whole Blood, with Reaction Vials
Order no.: 87000
480 Tests - Vitamin B1 (TPP, thiamine pyrophosphate)
- Vitamin B6 (PLP, pyridoxal 5’-phosphate)
Fast and easy sample preparation
Robust method with high selectivity
Short run time of 2.5 minutes
Isotopically-labelled internal standard for each analyte
With reaction vials
DESCRIPTION Clinical relevance Vitamin B1 (thiamine) plays an important role in deriving energy from carbohydrates and fats. The active form is thiamine pyrophosphate (TPP), a coenzyme for some enzymatic reactions in carbohydrate metabolism and for pyruvate decarboxylase. TPP is also significantly involved in the oxidative degradation of glucose. The determination of the TPP concentration is more significant than measurements of total thiamine, as by this method only the physiologically active form is taken into account. Vitamin B6 comprises pyridoxine, pyridoxamine and pyridoxal, as well as their phosphate derivatives. It is ingested with food and transferred via several enzymatic conversions into its active form, pyridoxal-5’-phosphate (PLP). PLP is involved in nearly one hundred different enzymatic reactions, among other things, as co-factor in amino acid metabolism, and in the formation of haemoglobin or neurotransmitters in the brain. Product advantages - Fast and easy sample preparation
- Robust method with high selectivity
- Short run time of 2.5 minutes
- Isotopically-labelled internal standard for each analyte
This Chromsystems assay enables combined determination of the active forms of vitamin B1 (thiamine pyrophosphate, TPP) and vitamin B6 (pyridoxal 5‘-phosphate, PLP) in whole blood by LC-MS/MS. Sample preparation consists of a simple protein precipitation step, with no need for derivatisation. This makes for a much faster sample preparation and high sample throughput. Analytes and potential interferences are effectively and swiftly separated within 2.5 minutes, indicating the method's high selectivity and speed. The chromatographic separation of the MassChrom® assay is fast enough to ensure a high throughput in the laboratory, but sufficiently resolving for a clear separation of the two analytes with minimal potential matrix effects. Isotope-labelled internal standards for both parameters as well as 6PLUS1® calibrators and MassCheck® controls ensure accurate and precise results. The assay is available in 2 configurations either with reaction vials or with 96 deep well plates. CHROMATOGRAM  TECHNICAL DATA Method of Analysis LC-MS/MS Number of Tests 480 Parameter Vitamin B1 (TPP, thiamine pyrophosphate), Vitamin B6 (PLP, pyridoxal 5’-phosphate) Analysis Time 2.5 min Limit of quantification 3.4 μg/l TPP
2.8 μg/l PLP Linearity 850 μg/l TPP
1000 μg/l PLP Intraassay CV = 1.9–5.2 % Interassay CV = 2.5–7.1 % Specimen Whole Blood Sample Preparation - Pipette 40 μl sample/calibrator/MassCheck® control into reaction vial.
- Add 80 μl Internal Standard Mix, mix briefly (vortex).
- Add 350 μl Precipitation Reagent, mix for 1 min (vortex) and centrifuge for 2 min.
- Transfer supernatant into autosampler vial.
- Inject 20 μl into the LC-MS/MS system.
Injection Volume 20 μl Flow rate 0.5 ml/min Column temperature 30 °C Ionisation ESI positive MS/MS-Mode MRM Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. KIT COMPONENTS Components available separately Mobile Phase A
Order no.: 87001 DETAILS Mobile Phase B
Order no.: 87002 DETAILS Internal Standard Set Vitamins B1 and B6
Order no.: 87046 DETAILS Precipitation Reagent
Order no.: 87005 DETAILS Rinsing Solution
Order no.: 87009 DETAILS Reaction Vials, 2 ml
Order no.: 33008 DETAILS ACCESSORIES PP Screw-on caps, pierceable silicone/PTFE septa, 1.0 mm
Order no.: J0410 DETAILS Autosampler vials, screw neck, amber glass, 1.5 ml
Order no.: J0601 DETAILS Analytical column
Order no.: 87100 DETAILS Tuning Mix Vitamins B1 and B6
Order no.: 87015 DETAILS Dilution Buffer
Order no.: 87018 DETAILS Adjustment Reagent
Order no.: 87019 DETAILS Autosampler vials, screw neck, 15 µl inner cone, clear glass, 1.1 ml
Order no.: J0602 DETAILS PE Screw-on caps, rubber/PTFE septa, 9 mm
Order no.: J0504 DETAILS Micro-inserts for autosampler vials
Order no.: J0403 DETAILS CALIBRATORS Calibrators available separately 6PLUS1® Multilevel Whole Blood Calibrator Set Vitamins B1 and B6
Order no.: 87039 DETAILS CONTROLS Controls available separately MassCheck® Vitamins B1 and B6 Whole Blood Controls
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